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PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA

Summary

Organism

Homo sapiens (human)

Reactome

R-HSA-9954714

PubChem

R-HSA-9954714

Description

Non-stop mRNAs (mRNAs that lack a stop codon) result in ribosomal stalls proximal to the 3' end of the mRNA, which are resolved by a distinct pathway. In this case, a complex comprising PELO, a paralog of the ribosome release factor eRF1, and HBS1L:GTP, a paralog of the ribosome release factor eRF3:GTP, binds the stalled ribosome near the subunit interface and the mRNA entry site (Shao et al. 2013, and inferred from human PELO:HBS1L and rabbit ribosomes in Pisareva et al. 2011, inferred from yeast homologs DOM34:HBS1 in Shoemaker et al. 2010, Tsuboi et al. 2012, Guydosh and Green 2014, reviewed in Franckenberg et al. 2012). PELO:HBS1L preferentially acts on ribosomes that are bound to mRNAs that have fewer than 12 nucleotides extending 3' of the ribosomal P site (Pisareva et al. 2011).
HBS1L hydrolyzes GTP and dissociates from PELO and the ribosome, exposing a site on PELO to which ABCE1 binds. ABCE1 then hydrolyzes ATP to cause a conformational change that splits the ribosome into 40S and 60S subunits (Shao et al. 2013, Shao and Hegde 2014, and inferred from the yeast homologs DOM34:HBS1 and archaeal homologs in Becker et al. 2012, inferred from the yeast homologs in Saito et al. 2013). ABCE1 and possibly the mRNA remain bound to the 40S ribosomal subunit, while the peptidyl-tRNA remains bound to the 60S ribosomal subunit as in the ASCC-mediated rescue pathway (Becker et al. 2012, reviewed in Franckenberg et al. 2012).

Click on a node on the pathway to see its details. Glycoproteins are marked with a glycoprotein icon in their name.

SPV: a JavaScript Signaling Pathway Visualizer, Bioinformatics, 2018

Displaying entries **1 - 10** of 54 in total

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UniProt ID	Protein Name	Gene Symbol	Pathway Viewer
P05387	Large ribosomal subunit protein P2	D11S2243E RPLP2 RPP2	view
P05388	Large ribosomal subunit protein uL10	RPLP0	view
P08708	Small ribosomal subunit protein eS17	RPS17 RPS17L	view
P15880	Small ribosomal subunit protein uS5	RPS2 RPS4	view
P18077	Large ribosomal subunit protein eL33	GIG33 RPL35A	view
P18621	Large ribosomal subunit protein uL22	RPL17	view
P23396	Small ribosomal subunit protein uS3	OK/SW-cl.26 RPS3	view
P25398	Small ribosomal subunit protein eS12	RPS12	view
P26373	Large ribosomal subunit protein eL13	BBC1 OK/SW-cl.46 RPL13	view
P32969	Large ribosomal subunit protein uL6	OK/SW-cl.103 RPL9; RPL9P7; RPL9P8; RPL9P9	view

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